"""
This module provides the `OnlineSampler` class and supporting methods.
Objects of the class `OnlineSampler`, are samplers which load examples
"on the fly" rather than storing them all persistently in memory.
"""
from abc import ABCMeta
import os
import random
import numpy as np
from .sampler import Sampler
from ..targets import GenomicFeatures
[docs]class OnlineSampler(Sampler, metaclass=ABCMeta):
"""
A sampler in which training/validation/test data is constructed
from random sampling of the dataset for each batch passed to the
model. This form of sampling may alleviate the problem of loading an
extremely large dataset into memory when developing a new model.
Parameters
----------
reference_sequence : selene_sdk.sequences.Sequence
A reference sequence from which to create examples.
target_path : str
Path to tabix-indexed, compressed BED file (`*.bed.gz`) of genomic
coordinates mapped to the genomic features we want to predict.
features : list(str)
List of distinct features that we aim to predict.
seed : int, optional
Default is 436. Sets the random seed for sampling.
validation_holdout : list(str) or float, optional
Default is `['chr6', 'chr7']`. Holdout can be regional or
proportional. If regional, expects a list (e.g. `['X', 'Y']`).
Regions must match those specified in the first column of the
tabix-indexed BED file. If proportional, specify a percentage
between (0.0, 1.0). Typically 0.10 or 0.20.
test_holdout : list(str) or float, optional
Default is `['chr8', 'chr9']`. See documentation for
`validation_holdout` for additional information.
sequence_length : int, optional
Default is 1000. Model is trained on sequences of `sequence_length`
where genomic features are annotated to the center regions of
these sequences.
center_bin_to_predict : int, optional
Default is 200. Query the tabix-indexed file for a region of
length `center_bin_to_predict`.
feature_thresholds : float [0.0, 1.0], optional
Default is 0.5. The `feature_threshold` to pass to the
`GenomicFeatures` object.
mode : {'train', 'validate', 'test'}, optional
Default is `'train'`. The mode to run the sampler in.
save_datasets : list(str), optional
Default is `[]` the empty list. The list of modes for which we should
save the sampled data to file (e.g. `["test", "validate"]`).
output_dir : str or None, optional
Default is None. The path to the directory where we should
save sampled examples for a mode. If `save_datasets` is
a non-empty list, `output_dir` must be specified. If
the path in `output_dir` does not exist it will be created
automatically.
Attributes
----------
reference_sequence : selene_sdk.sequences.Sequence
The reference sequence that examples are created from.
target : selene_sdk.targets.Target
The `selene_sdk.targets.Target` object holding the features that we
would like to predict.
validation_holdout : list(str) or float
The samples to hold out for validating model performance. These
can be "regional" or "proportional". If regional, this is a list
of region names (e.g. `['chrX', 'chrY']`). These regions must
match those specified in the first column of the tabix-indexed
BED file. If proportional, this is the fraction of total samples
that will be held out.
test_holdout : list(str) or float
The samples to hold out for testing model performance. See the
documentation for `validation_holdout` for more details.
sequence_length : int
The length of the sequences to train the model on.
bin_radius : int
From the center of the sequence, the radius in which to detect
a feature annotation in order to include it as a sample's label.
surrounding_sequence_radius : int
The length of sequence falling outside of the feature detection
bin (i.e. `bin_radius`) center, but still within the
`sequence_length`.
modes : list(str)
The list of modes that the sampler can be run in.
mode : str
The current mode that the sampler is running in. Must be one of
the modes listed in `modes`.
Raises
------
ValueError
If `mode` is not a valid mode.
ValueError
If the parities of `sequence_length` and `center_bin_to_predict`
are not the same.
ValueError
If `sequence_length` is smaller than `center_bin_to_predict` is.
ValueError
If the types of `validation_holdout` and `test_holdout` are not
the same.
"""
STRAND_SIDES = ('+', '-')
"""
Defines the strands that features can be sampled from.
"""
def __init__(self,
reference_sequence,
target_path,
features,
seed=436,
validation_holdout=['chr6', 'chr7'],
test_holdout=['chr8', 'chr9'],
sequence_length=1001,
center_bin_to_predict=201,
feature_thresholds=0.5,
mode="train",
save_datasets=[],
output_dir=None):
"""
Creates a new `OnlineSampler` object.
"""
super(OnlineSampler, self).__init__(
features,
save_datasets=save_datasets,
output_dir=output_dir)
self.seed = seed
np.random.seed(self.seed)
random.seed(self.seed + 1)
if (sequence_length + center_bin_to_predict) % 2 != 0:
raise ValueError(
"Sequence length of {0} with a center bin length of {1} "
"is invalid. These 2 inputs should both be odd or both be "
"even.".format(
sequence_length, center_bin_to_predict))
surrounding_sequence_length = sequence_length - center_bin_to_predict
if surrounding_sequence_length < 0:
raise ValueError(
"Sequence length of {0} is less than the center bin "
"length of {1}.".format(
sequence_length, center_bin_to_predict))
# specifying a test holdout partition is optional
if test_holdout:
self.modes.append("test")
if isinstance(validation_holdout, (list,)) and \
isinstance(test_holdout, (list,)):
self.validation_holdout = [
str(c) for c in validation_holdout]
self.test_holdout = [str(c) for c in test_holdout]
self._holdout_type = "chromosome"
elif isinstance(validation_holdout, float) and \
isinstance(test_holdout, float):
self.validation_holdout = validation_holdout
self.test_holdout = test_holdout
self._holdout_type = "proportion"
else:
raise ValueError(
"Validation holdout and test holdout must have the "
"same type (list or float) but validation was "
"type {0} and test was type {1}".format(
type(validation_holdout), type(test_holdout)))
else:
self.test_holdout = None
if isinstance(validation_holdout, (list,)):
self.validation_holdout = [
str(c) for c in validation_holdout]
else:
self.validation_holdout = validation_holdout
if mode not in self.modes:
raise ValueError(
"Mode must be one of {0}. Input was '{1}'.".format(
self.modes, mode))
self.mode = mode
self.surrounding_sequence_radius = int(
surrounding_sequence_length / 2)
self.sequence_length = sequence_length
self.bin_radius = int(center_bin_to_predict / 2)
self._start_radius = self.bin_radius
if center_bin_to_predict % 2 == 0:
self._end_radius = self.bin_radius
else:
self._end_radius = self.bin_radius + 1
self.reference_sequence = reference_sequence
self.n_features = len(self._features)
self.target = GenomicFeatures(
target_path, self._features,
feature_thresholds=feature_thresholds)
self._save_filehandles = {}
[docs] def get_feature_from_index(self, index):
"""
Returns the feature corresponding to an index in the feature
vector.
Parameters
----------
index : int
The index of the feature to retrieve the name for.
Returns
-------
str
The name of the feature occurring at the specified index.
"""
return self.target.index_feature_dict[index]
[docs] def get_sequence_from_encoding(self, encoding):
"""
Gets the string sequence from the one-hot encoding
of the sequence.
Parameters
----------
encoding : numpy.ndarray
An :math:`L \\times N` array (where :math:`L` is the length
of the sequence and :math:`N` is the size of the sequence
type's alphabet) containing the one-hot encoding of the
sequence.
Returns
-------
str
The sequence of :math:`L` characters decoded from the input.
"""
return self.reference_sequence.encoding_to_sequence(encoding)
[docs] def save_dataset_to_file(self, mode, close_filehandle=False):
"""
Save samples for each partition (i.e. train/validate/test) to
disk.
Parameters
----------
mode : str
Must be one of the modes specified in `save_datasets` during
sampler initialization.
close_filehandle : bool, optional
Default is False. `close_filehandle=True` assumes that all
data corresponding to the input `mode` has been saved to
file and `save_dataset_to_file` will not be called with
`mode` again.
"""
if mode not in self._save_datasets:
return
samples = self._save_datasets[mode]
if mode not in self._save_filehandles:
self._save_filehandles[mode] = open(
os.path.join(self._output_dir,
"{0}_data.bed".format(mode)),
'w+')
file_handle = self._save_filehandles[mode]
while len(samples) > 0:
cols = samples.pop(0)
line = '\t'.join([str(c) for c in cols])
file_handle.write("{0}\n".format(line))
if close_filehandle:
file_handle.close()
[docs] def get_data_and_targets(self, batch_size, n_samples=None, mode=None):
"""
This method fetches a subset of the data from the sampler,
divided into batches. This method also allows the user to
specify what operating mode to run the sampler in when fetching
the data.
Parameters
----------
batch_size : int
The size of the batches to divide the data into.
n_samples : int or None, optional
Default is None. The total number of samples to retrieve.
If `n_samples` is None and the mode is `validate`, will
set `n_samples` to 32000; if the mode is `test`, will set
`n_samples` to 640000 if it is None. If the mode is `train`
you must have specified a value for `n_samples`.
mode : str, optional
Default is None. The mode to run the sampler in when
fetching the samples. See
`selene_sdk.samplers.IntervalsSampler.modes` for more
information. If None, will use the current mode `self.mode`.
Returns
-------
sequences_and_targets, targets_matrix : \
tuple(list(tuple(numpy.ndarray, numpy.ndarray)), numpy.ndarray)
Tuple containing the list of sequence-target pairs, as well
as a single matrix with all targets in the same order.
Note that `sequences_and_targets`'s sequence elements are of
the shape :math:`B \\times L \\times N` and its target
elements are of the shape :math:`B \\times F`, where
:math:`B` is `batch_size`, :math:`L` is the sequence length,
:math:`N` is the size of the sequence type's alphabet, and
:math:`F` is the number of features. Further,
`target_matrix` is of the shape :math:`S \\times F`, where
:math:`S =` `n_samples`.
"""
if mode is not None:
self.set_mode(mode)
else:
mode = self.mode
sequences_and_targets = []
if n_samples is None and mode == "validate":
n_samples = 32000
elif n_samples is None and mode == "test":
n_samples = 640000
n_batches = int(n_samples / batch_size)
for _ in range(n_batches):
inputs, targets = self.sample(batch_size)
sequences_and_targets.append((inputs, targets))
targets_mat = np.vstack([t for (s, t) in sequences_and_targets])
if mode in self._save_datasets:
self.save_dataset_to_file(mode, close_filehandle=True)
return sequences_and_targets, targets_mat
[docs] def get_dataset_in_batches(self, mode, batch_size, n_samples=None):
"""
This method returns a subset of the data for a specified run
mode, divided into mini-batches.
Parameters
----------
mode : {'test', 'validate'}
The mode to run the sampler in when fetching the samples.
See `selene_sdk.samplers.IntervalsSampler.modes` for more
information.
batch_size : int
The size of the batches to divide the data into.
n_samples : int or None, optional
Default is `None`. The total number of samples to retrieve.
If `None`, it will retrieve 32000 samples if `mode` is validate
or 640000 samples if `mode` is test or train.
Returns
-------
sequences_and_targets, targets_matrix : \
tuple(list(tuple(numpy.ndarray, numpy.ndarray)), numpy.ndarray)
Tuple containing the list of sequence-target pairs, as well
as a single matrix with all targets in the same order.
The list is length :math:`S`, where :math:`S =` `n_samples`.
Note that `sequences_and_targets`'s sequence elements are of
the shape :math:`B \\times L \\times N` and its target
elements are of the shape :math:`B \\times F`, where
:math:`B` is `batch_size`, :math:`L` is the sequence length,
:math:`N` is the size of the sequence type's alphabet, and
:math:`F` is the number of features. Further,
`target_matrix` is of the shape :math:`S \\times F`
"""
return self.get_data_and_targets(
batch_size, n_samples=n_samples, mode=mode)
[docs] def get_validation_set(self, batch_size, n_samples=None):
"""
This method returns a subset of validation data from the
sampler, divided into batches.
Parameters
----------
batch_size : int
The size of the batches to divide the data into.
n_samples : int or None, optional
Default is `None`. The total number of validation examples
to retrieve. If `None`, 32000 examples are retrieved.
Returns
-------
sequences_and_targets, targets_matrix : \
tuple(list(tuple(numpy.ndarray, numpy.ndarray)), numpy.ndarray)
Tuple containing the list of sequence-target pairs, as well
as a single matrix with all targets in the same order.
Note that `sequences_and_targets`'s sequence elements are of
the shape :math:`B \\times L \\times N` and its target
elements are of the shape :math:`B \\times F`, where
:math:`B` is `batch_size`, :math:`L` is the sequence length,
:math:`N` is the size of the sequence type's alphabet, and
:math:`F` is the number of features. Further,
`target_matrix` is of the shape :math:`S \\times F`, where
:math:`S =` `n_samples`.
"""
return self.get_dataset_in_batches(
"validate", batch_size, n_samples=n_samples)
[docs] def get_test_set(self, batch_size, n_samples=None):
"""
This method returns a subset of testing data from the
sampler, divided into batches.
Parameters
----------
batch_size : int
The size of the batches to divide the data into.
n_samples : int or None, optional
Default is `None`. The total number of validation examples
to retrieve. If `None`, 640000 examples are retrieved.
Returns
-------
sequences_and_targets, targets_matrix : \
tuple(list(tuple(numpy.ndarray, numpy.ndarray)), numpy.ndarray)
Tuple containing the list of sequence-target pairs, as well
as a single matrix with all targets in the same order.
Note that `sequences_and_targets`'s sequence elements are of
the shape :math:`B \\times L \\times N` and its target
elements are of the shape :math:`B \\times F`, where
:math:`B` is `batch_size`, :math:`L` is the sequence length,
:math:`N` is the size of the sequence type's alphabet, and
:math:`F` is the number of features. Further,
`target_matrix` is of the shape :math:`S \\times F`, where
:math:`S =` `n_samples`.
Raises
------
ValueError
If no test partition of the data was specified during
sampler initialization.
"""
if "test" not in self.modes:
raise ValueError("No test partition of the data was specified "
"during initialization. Cannot use method "
"`get_test_set`.")
return self.get_dataset_in_batches("test", batch_size, n_samples)